Cell-cycle inhibition by Helicobacter pylori L-asparaginase.

Cell-cycle inhibition by Helicobacter pylori L-asparaginase.

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Helicobacter pylori (H.pylori) is a major human pathogen causing chronic gastritis, peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue lymphoma.One of the mechanisms whereby it induces damage depends on its interference with proliferation of host tissues.We here describe the discovery of a novel bacterial factor able to inhibit the cell-cycle 15-eg1053cl of exposed cells, both of gastric and non-gastric origin.An integrated approach was adopted to isolate and characterise the molecule from the bacterial culture filtrate produced in a protein-free medium: size-exclusion chromatography, non-reducing gel electrophoresis, mass spectrometry, mutant analysis, recombinant protein expression and enzymatic assays.

L-asparaginase was identified as the factor responsible for cell-cycle inhibition of fibroblasts and gastric cell lines.Its effect on cell-cycle was confirmed by inhibitors, a knockout strain and the action of recombinant L-asparaginase on cell lines.Interference with cell-cycle in vitro depended on cell genotype and was related to the expression levels of the concurrent enzyme asparagine synthetase.Bacterial subcellular distribution of L-asparaginase was also analysed along with its immunogenicity.H.

pylori L-asparaginase click here is a novel antigen that functions as a cell-cycle inhibitor of fibroblasts and gastric cell lines.We give evidence supporting a role in the pathogenesis of H.pylori-related diseases and discuss its potential diagnostic application.